Formulation and Activity Test of Breadfruit Leaves (Artocarpus altilis (Parkinson) Fosberg) Extract Serum Spray as Antioxidant

Abstract

Breadfruit leaves (Artocarpus altilis) contain bioactive flavonoids known to have antioxidant properties, which can be utilized in the formulation of skin care products. This study aims to develop and evaluate an antioxidant serum spray containing bread1fruit leaf extract. Breadfruit leaves extract was obtained through stepwise maceration using n-hexane, ethyl acetate, and 70% ethanol. Two spray serum formulas with different propylene glycol concentrations (5% in F1 and 3% in F2) were developed and tested for physical stability and antioxidant activity using DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Data were analyzed based on IC₅₀ values as well as stability tests including organoleptic, pH, viscosity, spray pattern, and adhesion spreadability. The IC₅₀ values for n-hexane, ethyl acetate, and ethanol extracts were 595.045 ppm, 47.466 ppm, and 129.177 ppm, respectively, showing significant antioxidant potential in the ethyl acetate extract. Spray serum containing ethyl acetate extract met the physical stability requirements regarding pH, viscosity, and spray pattern. Both formulas showed effective antioxidant activity, with The IC₅₀ values of 60.752 ppm (F1) and 71.918 ppm (F2), indicating the potential of the formulas to maintain antioxidant activity over time. The breadfruit leaf serum spray formulation showed strong potential as an antioxidant skin care product with stable physical properties.