Evaluation of Myo-inositol Influence on Andrographolide Production in Andrographis paniculata
Keywords:
Andrographis paniculata, andrographolide, myo-inositol, tissue culture, HPLCAbstract
Andrographis paniculata is widely recognized for its medicinal properties, especially andrographolide. This secondary metabolite has the potential to be antimicrobial, anti-inflammatory, and antidiabetic. This study aimed to enhance secondary metabolite production through tissue culture techniques by incorporating Myo-inositol into media formulations. The experimental setup involved germinating A. paniculata seeds under controlled conditions at 15°C with continuous illumination. Two media formulations were conducted: one supplemented with Myo-inositol and the other as a control. After germination and growth, the leaf, stem, and root tissues were harvested and macerated with methanol. Andrographolide concentrations were quantified using High-Performance Liquid Chromatography (HPLC), and statistical analysis was conducted using an independent samples t-test. The results revealed that the leaves contained the highest andrographolide concentration among the tissues analyzed. The andrographolide content in media without Myo-inositol was 4.072% and 3.052% with Myo-inositol. However, supplementing Myo-inositol in the media did not result in a statistically significant increase in andrographolide concentration compared to the control. These findings suggest that while the leaf tissue is a valuable source of andrographolide, the role of Myo-inositol in enhancing its production requires further investigation.
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